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BlueRAY Prestained Protein Ladder

10-180 kDa Colorimetric
产品编号 ABIN2868254
发货至: 中国
  • 检测方法
    Colorimetric
    检测范围
    10-180 kDa
    最低检测浓度
    10 kDa
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB)
  • 应用备注
    Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2-3 μL per well for general Western transfering. Apply more for thicker (>1.5mm) or larger gel.
    5 μL of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
    说明

    The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.Do not heat, dilute, add reducing agent before loading.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Tris-Glycine Bis-Tris 12 % Bis-Tris 10 % 4~20 % MOPS buffer MES buffer
    储存条件
    RT
    储存方法
    Store at 25°C for 6 months, Store at -20°C for 24 months
    有效期
    24 months
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