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- 检测方法
- Colorimetric
- 检测范围
- 10-180 kDa
- 最低检测浓度
- 10 kDa
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB)
- 原理
- The BlueAQUA Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa.
- 产品特性
- Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).The BlueAQUA Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
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BLUltra Prestained Protein Ladder
6.5-270 kDa Colorimetric
Protein Molecular Weight Marker14-120 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
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- 应用备注
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- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel. - 试剂准备
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Quality Control: Under suggested conditions, BlueAQUA Prestained Protein Ladder resolves 11 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Approximately 0.1~0.5 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
- 储存条件
- -20 °C
- 储存方法
- Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage, store at -20°C
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