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BLUeye Prestained Protein Ladder

10-245 kDa Colorimetric
产品编号 ABIN2868252
发货至: 中国
  • 检测方法
    Colorimetric
    检测范围
    10-245 kDa
    最低检测浓度
    10 kDa
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB)
  • 应用备注
    Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
    BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
    说明

    The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    试剂准备

    Quality Control:
    Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10 % Tris-Glycine Bis-Tris 10 % MES buffer 4~20 % MOPS buffer
    储存条件
    RT
    储存方法
    Store at 25°C for 6 months, Store at -20°C for 24 months
    有效期
    24 months
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