anti-人 ARAF 抗体 for Enzyme Immunoassay

Recommended ARAF Antibody (supplied by: Log in to see )

抗原
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) 抗体
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • A-Raf proto-oncogene, serine/threonine kinase
  • A-Raf proto-oncogene, serine/threonine kinase L homeolog
  • A-Raf proto-oncogene, serine/threonine kinase S homeolog
  • Araf proto-oncogene, serine/threonine kinase
  • araf
  • ARAF
  • araf.L
  • araf.S
  • Araf
抗原表位
Middle Region
24
23
16
15
11
10
10
10
9
7
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用

167
100
87
6
6
6
4
2
2
2
1
宿主

144
25
克隆类型
多克隆
标记
This ARAF antibody is un-conjugated
6
6
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
119
71
42
39
27
15
9
3
2
2
1
1
1
Supplier
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产品编号 ABIN360127
$ 390.50
加上运输费 $45.00

General

抗原 V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) 抗体
抗原表位 Middle Region
(24), (23), (16), (15), (11), (10), (10), (10), (9), (7), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(167), (100), (87), (6), (6), (6), (4), (2), (2), (2), (1)
宿主
(144), (25)
克隆类型
多克隆
标记 This ARAF antibody is un-conjugated
(6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(119), (71), (42), (39), (27), (15), (9), (3), (2), (2), (1), (1), (1)
Pubmed 4个引用可参考
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ARAF抗体详情

ARAF目标详情 使用细节 贮存及处理 References for anti-ARAF antibody (ABIN360127) 图像
特异性 This antibody reacts to A-RAF.
纯化方法 Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
免疫原 This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ARAF1.
亚型 Ig Fraction
Plasmids, Primers & others

ARAF目标详情

ARAF抗体详情 使用细节 贮存及处理 References for anti-ARAF antibody (ABIN360127) 图像 回页首
抗原
别名 ARAF (ARAF Antibody 摘要)
背景 Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
基因ID 369, 9606
UniProt P10398
途径 MAPK Pathway, RTK signaling, Hepatitis C

使用细节

ARAF抗体详情 ARAF目标详情 贮存及处理 References for anti-ARAF antibody (ABIN360127) 图像 回页首
应用备注 ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

ARAF抗体详情 ARAF目标详情 使用细节 References for anti-ARAF antibody (ABIN360127) 图像 回页首
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ARAF antibody (ABIN360127)

ARAF抗体详情 ARAF目标详情 使用细节 贮存及处理 图像 回页首
背景

Wu, Noh, Zhou, Dixon, Guan: "Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells." in: The Journal of biological chemistry, Vol. 271, Issue 6, pp. 3265-71, 1996

Lee, Beck, Brennscheidt, DeGennaro, Rapp: "The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1)." in: Genomics, Vol. 20, Issue 1, pp. 43-55, 1994

Popescu, Mark: "Localization of the pKs gene, a raf related sequence on human chromosomes X and 7." in: Oncogene, Vol. 4, Issue 4, pp. 517-9, 1989

Beck, Huleihel, Gunnell, Bonner, Rapp: "The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus." in: Nucleic acids research, Vol. 15, Issue 2, pp. 595-609, 1987

图像

ARAF抗体详情 ARAF目标详情 使用细节 贮存及处理 References for anti-ARAF antibody (ABIN360127) 回页首
厂商提供的图像
 image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody (ABIN360127) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Middle Region) antibody