Lung Tissue Lysate (Tumor)
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- 资源
- Human
- 应用范围
- Western Blotting (WB)
- 特异性
- Lung Tissue
- 产品特性
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Lung tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1 % Triton X-100, 1 % sodium deoxycholic acid, 0.1 % sodium dodecylsulfate, 5 μ,g/mL of aprotinin, 5 μ,g/mL of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % β,-mercaptoethanol.
Source: Human Lung Tissue
Age: 73
Gender: M
Race: Black
Diagnosis: Tumor
Cancer Type: Adenocarcinoma
Cancer Type (Details): Moderately differentiated
- Lysate Type
- Tumor Lysate
- Lysate Fraction
- Whole Tissue Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
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APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)IP, WB Whole Cell Lysate Cell Lysate THP-1 Cells 适用: 人
Rat liver tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
Rat brain tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
ZSCAN21 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)Zscan21 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
OLIG1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)OLIG1 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- SDS sample buffer containing 5 % β,-mercaptoethanol.
- 注意事项
- Repeated freezing and thawing is not recommended.
- 储存条件
- 4 °C/-80 °C
- 储存方法
- Store at 2-8˚,C for continuous use. For extended storage, freeze working aliquots at -70˚,C.
- 有效期
- 6 months
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