THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)

ABIN1339495 产品详细信息
宿主

资源
人细胞
应用范围
Immunoprecipitation (IP), Western Blotting (WB)
选项
原理 Whole cell lysate
特异性 Non-denatured
产品特性 THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)
Used Tissue: Peripheral Blood
Lysate Fraction Whole Cell Lysate
Lysate Type Cell Lysate
Lysed Cells THP-1 Cells
背景 Full Gene Name:
Synonyms:
应用备注 Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
说明

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

限制 仅限研究用
状态 Liquid
缓冲液 Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
注意事项 Aliquot to avoid repeated freezing and thawing.
储存条件 -80 °C
储存方法 Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
厂商提供的图像
 image for THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured) (ABIN1339495) THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)
有引用在: Massberg, Grahl, von Bruehl, Manukyan, Pfeiler, Goosmann, Brinkmann, Lorenz, Bidzhekov, Khandagale, Konrad, Kennerknecht, Reges, Holdenrieder, Braun, Reinhardt, Spannagl, Preissner, Engelmann: "Reciprocal coupling of coagulation and innate immunity via neutrophil serine proteases." in: Nature medicine, Vol. 16, Issue 8, pp. 887-96, 2010 (PubMed).