Lung Membrane Lysate
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- 资源
- Human
- 应用范围
- Western Blotting (WB)
- 产品特性
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Human lung tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human lung tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The membrane protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated lung tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated lung tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody to confirm there is no signal or very weak signal.
Source: Human Lung tissue
Diagnosis: Normal
Tissue or Cell type: Lung - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Membrane Fraction
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
THP-1 (human acute monocytic leukemia) whole cell lysate (non-denatured)IP, WB Whole Cell Lysate Cell Lysate THP-1 Cells 适用: 人
Rat liver tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
Rat brain tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
ZSCAN21 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)Zscan21 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
OLIG1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)OLIG1 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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- 应用备注
- Lung membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- 注意事项
- Repeated freezing and thawing is not recommended.
- 储存条件
- 4 °C/-80 °C
- 储存方法
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- 有效期
- 6 months
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