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Multiple Tag Cell Lysate

Name: Multiple Tag Cell Lysate
Brand: antibodies-online
SKU: ABIN1536505
Availability: OutOfStock

DB, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: E. coli

(1 reference)

产品编号 ABIN1536505

发货至: 中国

应用备注

Positive control for Western blot and Dot blot.

限制

仅限研究用
浓度

200 µg of whole E. coli cell lysate (lyophilized) Volume Add 200 µl of ddH2O to make the final of 1 µg/µl in 1X SDS Loading Buffer. 0.02 - 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buf

储存条件

-20 °C

储存方法

Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
Lawless, Mankan, White, ODwyer, Norris: "Expression of hereditary hemochromatosis C282Y HFE protein in HEK293 cells activates specific endoplasmic reticulum stress responses." in: BMC cell biology, Vol. 8, pp. 30, (2007) (PubMed).
背景

Epitope Tag Positive Control Lysates. Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.