小鼠 brain tissue lysate (non-denatured)
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- 宿主
- 小鼠
- 资源
- 小鼠
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 原理
- Whole tissue lysate
- 特异性
- Non-denatured
- 产品特性
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Mouse brain tissue lysate (non-denatured)
Used Tissue: Brain - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
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APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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Rat liver tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
Rat brain tissue lysate (non-denatured)IP, WB Whole Tissue Lysate Tissue Lysate 适用: 大鼠
ZSCAN21 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)Zscan21 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
OLIG1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)OLIG1 WB, IP Whole Cell Lysate Overexpression Lysate HEK 293T Cells 适用: 人
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- 应用备注
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- 说明
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Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- 背景
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Full Gene Name:
Synonyms:
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