RAF1 ELISA 试剂盒
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Quick Overview for RAF1 ELISA 试剂盒 (ABIN8088862)
抗原
See all RAF1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
应用范围
样品类型
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原理
- Phospho-C-RAF (Ser642) Colorimetric Cell-Based ELISA Kit for detecting Phospho-C-RAF (Ser642) in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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实验时间
- 4 - 5 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C for one month, Long term: see manual.
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有效期
- 6 months
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- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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别名
- C-RAF
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背景
- Gene Aliases: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
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基因ID
- 5894
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UniProt
- P04049
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途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
抗原 See all RAF1 ELISA试剂盒
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