RAF1 ELISA 试剂盒
RAF1
适用: 人, 小鼠, 大鼠
pSer301
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
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- 抗原 See all RAF1 ELISA试剂盒
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- 抗原表位
- pSer301, total
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适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human/Mouse/Rat Phospho-Raf-1 (Ser301) and Total Raf-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Raf-1 (Ser301) and Total Raf-1 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human, mouse, and rat Raf-1 phosphorylated at site Serine-301 and Total Raf-1.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product RAF1 ELISA Kit
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V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) ELISA Kit
RAF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately. - 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all RAF1 ELISA试剂盒
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- 别名
- Raf-1 (RAF1 产品)
- 别名
- CRAF ELISA Kit, NS5 ELISA Kit, Raf-1 ELISA Kit, c-Raf ELISA Kit, c-raf ELISA Kit, craf ELISA Kit, raf1 ELISA Kit, RAF1 ELISA Kit, 6430402F14Rik ELISA Kit, AA990557 ELISA Kit, BB129353 ELISA Kit, Craf1 ELISA Kit, D830050J10Rik ELISA Kit, v-Raf ELISA Kit, raf ELISA Kit, cRaf ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase a ELISA Kit, v-raf-leukemia viral oncogene 1 ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase S homeolog ELISA Kit, RAF1 ELISA Kit, Raf1 ELISA Kit, raf1a ELISA Kit, raf1 ELISA Kit, raf1.S ELISA Kit
- 背景
- RAF proto-oncogene serine/threonine-protein kinase (Raf-1) phosphorylated at Serine-301 and Total 301
- 基因ID
- 5894
- UniProt
- P04049
- 途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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