p53 ELISA 试剂盒
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Quick Overview for p53 ELISA 试剂盒 (ABIN8071210)
抗原
See all p53 (TP53) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.3125 ng/mL
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原理
- Ubiquitinated p53 Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse, Rat Ubiquitinated p53.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects Human, Mouse, Rat Ubiquitinated p53.
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灵敏度
- 0.07 ng/mL
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组件
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- Size / Qty
- Anti-Pan-p53 Microplate
- 96 wells
- Ubiquitinated-p53 Standard
- Anti-Ubiquitin Detection Antibody
- HRP-Streptavidin
- 200 μL
- Wash Buffer
- Assay Diluent B
- Lysis Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Log-log graph paper or computer and software for ELISA data analysis
- Absorbent paper
- Tubes to prepare positive control or sample dilutions
- Distilled or deionized water
- Shaker
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Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 acetylated Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 AA 1-393 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL of each standard and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
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Gene Symbols: TP53 P53
Background: The Ubiquitinated p53 Quantitative ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human, Mouse, Rat Ubiquitinated p53. An anti-pan p53 antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and p53 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-ubiquitin antibody is used to detect ubiquitinated p53. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ubiquitinated p53 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Protein Name (Synonyms): Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)
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基因ID
- 71572205924842
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UniProt
- P04637, P02340, P10361
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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