HRAS ELISA 试剂盒
The 人 HRAS ELISA Kit (ABIN7830972) is a Colorimetric ELISA Kit designed to quantify 人 HRAS.
产品编号 ABIN7830972
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for HRAS ELISA 试剂盒 (ABIN7830972)
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HRAS
(HRas proto-oncogene, GTPase (HRAS))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Adherent Cell Culture, Cell Culture Cells, Cell Samples
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原理
- HRAS/KRAS/NRAS (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HRAS/KRAS/NRAS expression in cultured cells.
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Analytical Method
- Qualitative
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HRas proto-oncogene, GTPase (HRAS) ELISA Kit
HRAS 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- HRAS (HRas proto-oncogene, GTPase (HRAS))
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别名
- HRAS
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背景
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Synonyms: C-BAS/HAS|C-H-RAS|C-HA-RAS1|CTLO|H-RASIDX|HAMSV|HRAS1|K-RAS|N-RAS|RASH1
Gene Description: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
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基因ID
- 3265
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途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling
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