NF-kB p65 ELISA 试剂盒
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Quick Overview for NF-kB p65 ELISA 试剂盒 (ABIN7830876)
抗原
See all NF-kB p65 (NFkBP65) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- RELA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RELA expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠
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Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 适用: 人 Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- RELA
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背景
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Synonyms: MGC131774|NFKB3|p65
Gene Description: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
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基因ID
- 5970
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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