RAF1 ELISA 试剂盒
This Colorimetric ELISA kit is designed for the quantitative measurement of 人 RAF1.
产品编号 ABIN7830524
发货至:
中国
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Our Local Distributor
中国
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Quick Overview for RAF1 ELISA 试剂盒 (ABIN7830524)
抗原
See all RAF1 ELISA试剂盒
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- RAF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RAF1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) ELISA Kit
RAF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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别名
- RAF1
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背景
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Synonyms: CRAF|NS5|Raf-1|c-Raf
Gene Description: v-raf-1 murine leukemia viral oncogene homolog 1
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基因ID
- 5894
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UniProt
- P04049
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途径
- MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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