TRKB ELISA 试剂盒
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- 抗原 See all TRKB (NTRK2) ELISA试剂盒
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
- 抗原表位
- pTyr816
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适用
- 人, 大鼠, 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-TrkB (Tyr816) ELISA Kit. This assay semi-quantitatively measures TrkB phosphorylated at Tyrosine-816 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibodies provided in this kit recognizes human, mouse, and rat TrkB phosphorylated at Tyrosine-816.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our best selling NTRK2 ELISA Kit
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- Discover our top product NTRK2 ELISA Kit
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Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kit
NTRK2 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 小鼠 AA 32-429 Colorimetric Sandwich ELISA 93.8-6000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA KitNTRK2 适用: 人 AA 32-430 Colorimetric Sandwich ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all TRKB (NTRK2) ELISA试剂盒
- TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
- 别名
- TrkB (NTRK2 产品)
- 别名
- trkb ELISA Kit, trkb-b ELISA Kit, xtrkb ELISA Kit, si:ch211-220b11.1 ELISA Kit, trkB2 ELISA Kit, NTRK2 ELISA Kit, ntrk2 ELISA Kit, gp145-trkb ELISA Kit, TRKB ELISA Kit, GP145-TrkB ELISA Kit, trk-B ELISA Kit, AI848316 ELISA Kit, C030027L06Rik ELISA Kit, Tkrb ELISA Kit, trkB ELISA Kit, RATTRKB1 ELISA Kit, TRKB1 ELISA Kit, Trk-B ELISA Kit, TrkB ELISA Kit, neurotrophic receptor tyrosine kinase 2 ELISA Kit, neurotrophic receptor tyrosine kinase 2 L homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2b ELISA Kit, neurotrophic receptor tyrosine kinase 2 S homeolog ELISA Kit, neurotrophic tyrosine kinase, receptor, type 2 ELISA Kit, NTRK2 ELISA Kit, ntrk2.L ELISA Kit, ntrk2b ELISA Kit, ntrk2.S ELISA Kit, ntrk2 ELISA Kit, Ntrk2 ELISA Kit
- 基因ID
- 4915
- 途径
- RTK signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
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