Ephrin A1 ELISA 试剂盒
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- 抗原 See all Ephrin A1 (EFNA1) ELISA试剂盒
- Ephrin A1 (EFNA1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human EFNA1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product EFNA1 ELISA Kit
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Ephrin A1 (EFNA1) ELISA Kit
EFNA1 适用: 小鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Ephrin A1 (EFNA1) ELISA KitEFNA1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Ephrin A1 (EFNA1) ELISA KitEFNA1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all Ephrin A1 (EFNA1) ELISA试剂盒
- Ephrin A1 (EFNA1)
- 别名
- EFNA1 (EFNA1 产品)
- 别名
- Xefrin-A1 ELISA Kit, b61 ELISA Kit, ecklg ELISA Kit, efl1 ELISA Kit, efna1 ELISA Kit, efna1a ELISA Kit, elfa-a ELISA Kit, epha1 ELISA Kit, eplg1 ELISA Kit, lerk1 ELISA Kit, tnfaip4 ELISA Kit, xelf-a' ELISA Kit, xelfa ELISA Kit, AI325262 ELISA Kit, B61 ELISA Kit, Efl1 ELISA Kit, Epl1 ELISA Kit, Eplg1 ELISA Kit, Lerk1 ELISA Kit, ECKLG ELISA Kit, EFL1 ELISA Kit, EPLG1 ELISA Kit, LERK-1 ELISA Kit, LERK1 ELISA Kit, TNFAIP4 ELISA Kit, efna1b ELISA Kit, MGC130661 ELISA Kit, ephrin-A1 S homeolog ELISA Kit, ephrin-A1 ELISA Kit, ephrin A1 ELISA Kit, ephrin-A1b ELISA Kit, ephrin-A1 L homeolog ELISA Kit, efna1.S ELISA Kit, efna1 ELISA Kit, EFNA1 ELISA Kit, efna1b ELISA Kit, Efna1 ELISA Kit, efna1.L ELISA Kit
- 基因ID
- 1942
- UniProt
- P20827
- 途径
- RTK signaling
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