CRADD ELISA 试剂盒
CRADD
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CRADD ELISA试剂盒
- CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CRADD ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CRADD ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all CRADD ELISA试剂盒
- CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))
- 别名
- CRADD (CRADD 产品)
- 别名
- cradd ELISA Kit, MGC89035 ELISA Kit, zgc:101717 ELISA Kit, CRADD ELISA Kit, DKFZp468A2219 ELISA Kit, LOC100226369 ELISA Kit, raidd ELISA Kit, MGC78788 ELISA Kit, MRT34 ELISA Kit, RAIDD ELISA Kit, CASP2 and RIPK1 domain containing adaptor with death domain ELISA Kit, CASP2 and RIPK1 domain containing adaptor with death, gene 1 ELISA Kit, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog ELISA Kit, CRADD ELISA Kit, cradd.1 ELISA Kit, cradd ELISA Kit, cradd.2.S ELISA Kit, Cradd ELISA Kit
- 基因ID
- 8738
- UniProt
- P78560
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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