FCER2 ELISA 试剂盒
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- 抗原 See all FCER2 ELISA试剂盒
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse FCER2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse FCER2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product FCER2 ELISA Kit
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Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA Kit
FCER2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 AA 48-321 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 小鼠 AA 50-331 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all FCER2 ELISA试剂盒
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
- 别名
- FCER2 (FCER2 产品)
- 别名
- BLAST-2 ELISA Kit, CD23 ELISA Kit, CD23A ELISA Kit, CLEC4J ELISA Kit, FCE2 ELISA Kit, IGEBF ELISA Kit, Fcer2a ELISA Kit, Fc fragment of IgE receptor II ELISA Kit, FCER2 ELISA Kit, Fcer2 ELISA Kit
- UniProt
- P20693
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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