FCER2 ELISA 试剂盒
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Quick Overview for FCER2 ELISA 试剂盒 (ABIN3044698)
抗原
See all FCER2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD23/FCER2
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: E50-P331 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <15pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
- Immunogen sequence: E50-P331
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Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA Kit
FCER2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 AA 48-321 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) ELISA KitFCER2 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD23 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E50-P331) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD23 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD23 standard solution and each sample be measured in duplicate.
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限制
- 仅限研究用
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缓冲液
- heparin or EDTA
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
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别名
- FCER2
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背景
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Protein Function: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Background: CD23, also known as Fc epsilon RII, or FciμRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
Synonyms: Low affinity immunoglobulin epsilon Fc receptor,Fc-epsilon-RII,Lymphocyte IgE receptor,CD23,Fcer2,Fcer2a,
Full Gene Name: Low affinity immunoglobulin epsilon Fc receptor
Cellular Localisation: Cell membrane, Single-pass type II membrane protein . Cell membrane, Lipid-anchor. -
基因ID
- 14128
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UniProt
- P20693
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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