ERK2 ELISA 试剂盒
MAPK1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ERK2 (MAPK1) ELISA试剂盒
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human MAPK1/ERK2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human MAPK1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product MAPK1 ELISA Kit
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Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA Kit
MAPK1 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ERK Phosphorylation Assay KitBCA 适用: 人, 大鼠, 小鼠
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ERK2 (MAPK1) ELISA试剂盒
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
- 别名
- MAPK1 (ERK2 (MAPK1 产品)
- 别名
- ERK-1 ELISA Kit, ERK1 ELISA Kit, ERT2 ELISA Kit, HS44KDAP ELISA Kit, HUMKER1A ELISA Kit, P44ERK1 ELISA Kit, P44MAPK ELISA Kit, PRKM3 ELISA Kit, p44-ERK1 ELISA Kit, p44-MAPK ELISA Kit, Erk-1 ELISA Kit, Erk1 ELISA Kit, Ert2 ELISA Kit, Esrk1 ELISA Kit, Mnk1 ELISA Kit, Mtap2k ELISA Kit, Prkm3 ELISA Kit, p44 ELISA Kit, p44erk1 ELISA Kit, p44mapk ELISA Kit, ERK ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, 9030612K14Rik ELISA Kit, AA407128 ELISA Kit, AU018647 ELISA Kit, C78273 ELISA Kit, Erk2 ELISA Kit, Prkm1 ELISA Kit, p42mapk ELISA Kit, zERK2 ELISA Kit, MAPK1 ELISA Kit, Xp42 ELISA Kit, erk ELISA Kit, erk2 ELISA Kit, ert1 ELISA Kit, mapk ELISA Kit, mapk1b ELISA Kit, mapk2 ELISA Kit, mpk1 ELISA Kit, prkm1 ELISA Kit, prkm2 ELISA Kit, mapk1 ELISA Kit, mapk1a ELISA Kit, xp42 ELISA Kit, ATMPK1 ELISA Kit, F14N23.9 ELISA Kit, F14N23_9 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, MNK1 ELISA Kit, ATMPK2 ELISA Kit, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 ELISA Kit, T30E16.13 ELISA Kit, T30E16_13 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, mitogen activated protein kinase 1 ELISA Kit, mitogen-activated protein kinase 1 L homeolog ELISA Kit, mitogen-activated protein kinase 1 S homeolog ELISA Kit, mitogen activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase homolog 2 ELISA Kit, MAPK3 ELISA Kit, Mapk3 ELISA Kit, MAPK1 ELISA Kit, Mapk1 ELISA Kit, mapk1 ELISA Kit, mapk1.L ELISA Kit, mapk1.S ELISA Kit, PfMAP1 ELISA Kit, MPK1 ELISA Kit, PVX_084965 ELISA Kit, LOC9328617 ELISA Kit, Tsp_01601 ELISA Kit, MPK2 ELISA Kit
- UniProt
- P28482
- 途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
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