ERK2 ELISA 试剂盒
Quick Overview for ERK2 ELISA 试剂盒 (ABIN6730481)
抗原
See all ERK2 (MAPK1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- Human MAPK1/ERK2 ELISA Kit.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair recognizes Human MAPK1.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA Kit
MAPK1 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase 1 (MAPK1) ELISA KitMAPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ERK Phosphorylation Assay KitBCA 适用: 人, 大鼠, 小鼠
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
-
别名
- MAPK1 (ERK2
-
UniProt
- P28482
-
途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原 See all ERK2 (MAPK1) ELISA试剂盒
-
