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TRKA ELISA 试剂盒

NTRK1 适用: 大鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Lysate
产品编号 ABIN6719879
发货至: 中国
  • 抗原 See all TRKA (NTRK1) ELISA试剂盒
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    适用
    • 7
    • 6
    • 5
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156 pg/mL - 10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Lysate, Cell Culture Supernatant, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性

    Synonyms: High affinity nerve growth factor receptor, MTC, Neurotrophic tyrosine kinase receptor type 1, NTRK1, NTRK1_HUMAN, Oncogene TRK, p140-TrkA, TRK, Trk-A, TRK1, TRK1-transforming tyrosine kinase protein

    Background: Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

    Gene Name: NTRK1

    Production: Natural and recombinant rat TrkA. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: A33-P418

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  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat TrkA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue lysates to each empty well. It is recommended that each rat TrkA standard solution and each sample be measured in duplicate.

    ELISA Dilution: 156pg/mL-10000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all TRKA (NTRK1) ELISA试剂盒
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    别名
    TrkA (NTRK1 产品)
    别名
    MTC ELISA Kit, TRK ELISA Kit, TRK1 ELISA Kit, TRKA ELISA Kit, Trk-A ELISA Kit, p140-TrkA ELISA Kit, CDHF12 ELISA Kit, CDHR16 ELISA Kit, HSCR1 ELISA Kit, MEN2A ELISA Kit, MEN2B ELISA Kit, MTC1 ELISA Kit, PTC ELISA Kit, RET-ELE1 ELISA Kit, RET51 ELISA Kit, Trk ELISA Kit, C80751 ELISA Kit, Tkr ELISA Kit, TrkA ELISA Kit, trk ELISA Kit, trka ELISA Kit, CTRKA ELISA Kit, trkA ELISA Kit, neurotrophic receptor tyrosine kinase 1 ELISA Kit, ret proto-oncogene ELISA Kit, neurotrophic tyrosine kinase, receptor, type 1 ELISA Kit, NTRK1 ELISA Kit, RET ELISA Kit, Ntrk1 ELISA Kit, ntrk1 ELISA Kit
    基因ID
    59109
    NCBI登录号
    NP_067600
    UniProt
    P35739
    途径
    RTK signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process
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