MMP 9 IQ-ELISA 试剂盒
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- 抗原 See all MMP 9 (MMP9) IQ-ELISA Kits
- MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
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适用
- 人
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) MMP-9 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 灵敏度
- 1 pg/mL
- 产品特性
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- 组件
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- MMP-9 Microplate (Item A): 96 well PCR plate coated with anti-Human MMP-9
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human MMP-9
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for MMP-9 (Item F): 2 vials of a 4x concentrated solution of anti-Human MMP-9 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
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Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) ELISA KitMMP9 适用: 人 AA 20-707 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9) ELISA KitMMP9 适用: 小鼠 AA 20-730 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- 样本量
- 25 µL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all MMP 9 (MMP9) IQ-ELISA Kits
- MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
- 别名
- MMP-9 (MMP9 产品)
- 别名
- CLG4B IQ-ELISA Kit, GELB IQ-ELISA Kit, MANDP2 IQ-ELISA Kit, MMP-9 IQ-ELISA Kit, AW743869 IQ-ELISA Kit, B/MMP9 IQ-ELISA Kit, Clg4b IQ-ELISA Kit, pro-MMP-9 IQ-ELISA Kit, mmp-9 IQ-ELISA Kit, fgMMP-9 IQ-ELISA Kit, mmp9 IQ-ELISA Kit, ZFMMP-9 IQ-ELISA Kit, wu:fb02g06 IQ-ELISA Kit, wu:fb07b05 IQ-ELISA Kit, wu:fi98c09 IQ-ELISA Kit, wu:fj05a08 IQ-ELISA Kit, zgc:64165 IQ-ELISA Kit, clg4b IQ-ELISA Kit, gelb IQ-ELISA Kit, mandp2 IQ-ELISA Kit, matrix metallopeptidase 9 IQ-ELISA Kit, collagenase IQ-ELISA Kit, matrix metalloproteinase 9 IQ-ELISA Kit, matrix metallopeptidase 9 S homeolog IQ-ELISA Kit, MMP9 IQ-ELISA Kit, RB3913 IQ-ELISA Kit, BPSS0666 IQ-ELISA Kit, Sbal_3732 IQ-ELISA Kit, Bcer98_0486 IQ-ELISA Kit, Shew185_0630 IQ-ELISA Kit, Shal_3056 IQ-ELISA Kit, Sbal195_0657 IQ-ELISA Kit, Lbys_3550 IQ-ELISA Kit, Palpr_2084 IQ-ELISA Kit, Mmp9 IQ-ELISA Kit, mmp9 IQ-ELISA Kit, mmp9.S IQ-ELISA Kit
- 基因ID
- 4318
- UniProt
- P14780
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
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