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MMP 9 ELISA 试剂盒

MMP9 适用: 小鼠 AA 20-730 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
产品编号 ABIN411334
发货至: 中国
  • 抗原 See all MMP 9 (MMP9) ELISA试剂盒
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    抗原表位
    AA 20-730
    适用
    • 12
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156-10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-9
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: CHO
    Immunogen sequence: A20-P730
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <20pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: CHO
    Immunogen sequence: A20-P730
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the peptidase M10A family.

    板类型
    Pre-coated
    实验流程
    mouse MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-9 has been precoated onto 96-well plates. Standards(CHO, A20-P730) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-9 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MMP-9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MMP-9 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 1124, Standard deviation: 65.2, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 3855, Standard deviation: 181.2, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 6245, Standard deviation: 381, CV(%): 6.1,
    • Sample 1: n=24, Mean(pg/ml): 1376, Standard deviation: 89.44, CV(%): 6.5
    • Sample 2: n=24, Mean(pg/ml): 3921, Standard deviation: 204, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 6534, Standard deviation: 490, CV(%): 7.5
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • Zou, Wang, Liu, Bai, Wang, Liu, Zhang, Xiao, Wang: "Nucleolin mediated pro-angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post-transcriptional regulation of VEGF-A and MMP-9." in: Journal of cellular and molecular medicine, Vol. 22, Issue 5, pp. 2692-2705, (2018) (PubMed).

    Fischer, Doehn, Herr, Lachner, Heinrich, Kershaw, Voss, Jacobson, Gruber, Clauss, Witzenrath, Bals, Gutbier, Slevogt: "Acute Moraxella catarrhalis Airway Infection of Chronically Smoke-Exposed Mice Increases Mechanisms of Emphysema Development: A Pilot Study." in: European journal of microbiology & immunology, Vol. 8, Issue 4, pp. 128-134, (2018) (PubMed).

    Bai, Yin, Feng, Cao, Wu, Zhu, Li, Tu, Chai: "Corydalis hendersonii Hemsl. protects against myocardial injury by attenuating inflammation and fibrosis via NF-κB and JAK2-STAT3 signaling pathways." in: Journal of ethnopharmacology, Vol. 207, pp. 174-183, (2017) (PubMed).

    Chen, Zeng, Zhan, Wang, Jiang, Li: "Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-mediated paracrine Wnt10b." in: Oncogene, Vol. 36, Issue 33, pp. 4692-4705, (2017) (PubMed).

    Dai, Ji, Jiang, Sun: "Marsdenia tenacissima extract suppresses tumor growth and angiogenesis in A20 mouse lymphoma." in: Oncology letters, Vol. 13, Issue 5, pp. 2897-2902, (2017) (PubMed).

    Frati, Ricci, Pierucci, Nistri, Bani, Meacci: "Role of sphingosine kinase/S1P axis in ECM remodeling of cardiac cells elicited by relaxin." in: Molecular endocrinology (Baltimore, Md.), Vol. 29, Issue 1, pp. 53-67, (2016) (PubMed).

    Papakonstantinou, Karakiulakis, Batzios, Savic, Roth, Tamm, Stolz: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).

    Aragon, Erdely, Bishop, Salmen, Weaver, Liu, Hall, Eye, Kodali, Zeidler-Erdely, Stafflinger, Ottens, Campen: "MMP-9-Dependent Serum-Borne Bioactivity Caused by Multiwalled Carbon Nanotube Exposure Induces Vascular Dysfunction via the CD36 Scavenger Receptor." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 150, Issue 2, pp. 488-98, (2016) (PubMed).

    Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

    Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

    Jiang, Han, Li, Li, Yang, Liu: "Preparation and anti-tumor metastasis of carboxymethyl chitosan." in: Carbohydrate polymers, Vol. 125, pp. 53-60, (2015) (PubMed).

    Chen, Yao, Zhu, Li, Guo, Yu: "Expression levels of matrix metalloproteinase-9 in human gastric carcinoma." in: Oncology letters, Vol. 9, Issue 2, pp. 915-919, (2015) (PubMed).

    Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

    Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

    Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." in: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

    Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." in: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

    Guo, Wu, Cen, Li, Kong, Zhou: "IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis." in: Virology journal, Vol. 11, Issue 1, pp. 2511, (2014) (PubMed).

    Yuan, Zhan, Duan, Song, Zeng, Chen, Yang, Xia: "A functional polymorphism at miR-491-5p binding site in the 3'-UTR of MMP-9 gene confers increased risk for atherosclerotic cerebral infarction in a Chinese population." in: Atherosclerosis, Vol. 226, Issue 2, pp. 447-52, (2013) (PubMed).

    Tang, Tang, Fang, Liang, Zhang: "Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells." in: World journal of gastroenterology, Vol. 19, Issue 10, pp. 1582-92, (2013) (PubMed).

    Kang, Li, Wei, Zhang, Bian, Wang, Wu, Hu, Li, Yang: "Elevation of matrix metalloproteinase-9 level in cerebrospinal fluid of tick-borne encephalitis patients is associated with IgG extravassation and disease severity." in: PLoS ONE, Vol. 8, Issue 11, pp. e77427, (2013) (PubMed).

  • 抗原 See all MMP 9 (MMP9) ELISA试剂盒
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    别名
    MMP9 (MMP9 产品)
    别名
    CLG4B ELISA Kit, GELB ELISA Kit, MANDP2 ELISA Kit, MMP-9 ELISA Kit, AW743869 ELISA Kit, B/MMP9 ELISA Kit, Clg4b ELISA Kit, pro-MMP-9 ELISA Kit, mmp-9 ELISA Kit, fgMMP-9 ELISA Kit, mmp9 ELISA Kit, ZFMMP-9 ELISA Kit, wu:fb02g06 ELISA Kit, wu:fb07b05 ELISA Kit, wu:fi98c09 ELISA Kit, wu:fj05a08 ELISA Kit, zgc:64165 ELISA Kit, clg4b ELISA Kit, gelb ELISA Kit, mandp2 ELISA Kit, matrix metallopeptidase 9 ELISA Kit, collagenase ELISA Kit, matrix metalloproteinase 9 ELISA Kit, matrix metallopeptidase 9 S homeolog ELISA Kit, MMP9 ELISA Kit, RB3913 ELISA Kit, BPSS0666 ELISA Kit, Sbal_3732 ELISA Kit, Bcer98_0486 ELISA Kit, Shew185_0630 ELISA Kit, Shal_3056 ELISA Kit, Sbal195_0657 ELISA Kit, Lbys_3550 ELISA Kit, Palpr_2084 ELISA Kit, Mmp9 ELISA Kit, mmp9 ELISA Kit, mmp9.S ELISA Kit
    背景

    Protein Function: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity). .

    Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92- kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

    Synonyms: Matrix metalloproteinase-9,MMP-9,3.4.24.35,92 kDa gelatinase,92 kDa type IV collagenase,Gelatinase B,GELB,Mmp9,Clg4b,

    Full Gene Name: Matrix metalloproteinase-9

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.
    基因ID
    17395
    UniProt
    P41245
    途径
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
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