Selectin E/CD62e ELISA 试剂盒
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- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 10-3000 pg/mL
- 最低检测浓度
- 10 pg/mL
- 应用范围
- ELISA
- 原理
- Mouse E-Selectin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
- 灵敏度
- 10 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product SELE ELISA Kit
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Selectin E (SELE) ELISA Kit
SELE 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Selectin E (SELE) ELISA KitSELE 适用: 犬 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 AA 22-556 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Soluble Colorimetric 78-5000 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Soluble Colorimetric Sandwich ELISA 93.50-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples200 - 2,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 别名
- E-Selectin (SELE 产品)
- 别名
- SELE ELISA Kit, CD62E ELISA Kit, ELAM ELISA Kit, ELAM1 ELISA Kit, ESEL ELISA Kit, LECAM2 ELISA Kit, E-selectin ELISA Kit, Elam ELISA Kit, selectin E ELISA Kit, selectin, endothelial cell ELISA Kit, SELE ELISA Kit, Sele ELISA Kit
- 背景
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Gene Names: Sele Elam-1
Protein names: E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
- 基因ID
- 20339
- UniProt
- Q00690
- 途径
- Thromboxane A2 Receptor Signaling
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