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Selectin E/CD62e ELISA 试剂盒

Name: Selectin E/CD62e ELISA 试剂盒
SKU: ABIN411350
Availability: OutOfStock

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 Selectin E/CD62e. There are 8 publications available.

ELISA

1 image

(8 references)

产品编号 ABIN411350

发货至: 中国

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Quick Overview for Selectin E/CD62e ELISA 试剂盒 (ABIN411350)

抗原

See all Selectin E/CD62e (SELE) ELISA试剂盒

Selectin E/CD62e (SELE) (Selectin E (SELE))

抗原表位

AA 22-556

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

125 pg/mL - 8000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Discover our top product SELE ELISA Kit

最低检测浓度

125 pg/mL

原理

Sandwich Human sE-Selectin / CD62E ELISA Kit to quantitate Human SELE in cell culture supernatants, serum and plasma (heparin, citrate).

Analytical Method

Quantitative

特异性

Expression system for standard: NS0
Immunogen sequence: W22-P556
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 4 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Selectin E (SELE) ELISA Kit
SELE 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Selectin E (SELE) ELISA Kit
SELE 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 7.9%, 6%, 5.9%
Inter-Assay Precision (CV%): 8%, 7.1%, 7.4%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Dehghan, Sabri, Javanmard, Ahmadi, Mansourian: "Neurally mediated syncope: Is it really an endothelial dysfunction?" in: Anatolian journal of cardiology, Vol. 16, Issue 9, pp. 701-6, (2018) (PubMed).

Ahluwalia, Misto, Vozzi, Magliaro, Mattei, Marescotti, Avogaro, Iori: "Systemic and vascular inflammation in an in-vitro model of central obesity." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192824, (2018) (PubMed).

Park, Willoughby, Song, Leutholtz, Koh: "Exercise-induced changes in stress hormones and cell adhesion molecules in obese men." in: Journal of inflammation research, Vol. 11, pp. 69-75, (2018) (PubMed).

Majidinia, Rasmi, Khadem Ansari, Seyed-Mohammadzad, Saboory, Shirpoor: "Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X." in: Iranian journal of pharmaceutical research : IJPR, Vol. 15, Issue 3, pp. 561-566, (2016) (PubMed).

Bilgic, Yilmaz, Bozkurt, Celik, Bilgic, Gurel, Kirbas, Bavbek, Akcay: "Relationship of late arteriovenous fistula stenosis with soluble E-selectin and soluble EPCR in chronic hemodialysis patients with arteriovenous fistula." in: Clinical and experimental nephrology, Vol. 19, Issue 1, pp. 133-9, (2015) (PubMed).

Sabri, Dehghan, Yaghini, Nasiri, Mansourian, Khalifehsoltani: "Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 20, Issue 8, pp. 771-6, (2015) (PubMed).

Asgary, Keshvari, Sahebkar, Hashemi, Rafieian-Kopaei: "Clinical investigation of the acute effects of pomegranate juice on blood pressure and endothelial function in hypertensive individuals." in: ARYA atherosclerosis, Vol. 9, Issue 6, pp. 326-31, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." in: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).
抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒

Selectin E/CD62e (SELE) (Selectin E (SELE))

别名

SELE

背景

Background: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.
Gene Full Name: selectin E

基因ID

6401

UniProt

P16581

途径

Thromboxane A2 Receptor Signaling