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ACVRL1 ELISA 试剂盒
ACVRL1
适用: 小鼠
Colorimetric
Sandwich ELISA
0.41-100 ng/mL
Cell Culture Supernatant, Plasma, Serum
ACVRL1ELISA Kit详情
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ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
适用
All reactivities for ACVRL1 ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.41-100 ng/mL
最低检测浓度
0.41 ng/mL
应用范围
ELISA
原理
Mouse ALK-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
ACVRL1目标详情
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抗原
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ACVRL1
(Activin A Receptor Type II-Like 1 (ACVRL1))
别名
ALK-1 (ACVRL1 产品 )
别名
ACVRL1 ELISA Kit, vgb ELISA Kit, alk1 ELISA Kit, zehn1109 ELISA Kit, hm:zehn1109 ELISA Kit, ACVRLK1 ELISA Kit, ALK-1 ELISA Kit, ALK1 ELISA Kit, HHT ELISA Kit, HHT2 ELISA Kit, ORW2 ELISA Kit, SKR3 ELISA Kit, TSR-I ELISA Kit, R-3 ELISA Kit, R3 ELISA Kit, SETHKIR ELISA Kit, AI115505 ELISA Kit, AI427544 ELISA Kit, Acvrlk1 ELISA Kit, Alk1 ELISA Kit, activin A receptor like type 1 ELISA Kit, activin A receptor type II-like 1 ELISA Kit, activin A receptor, type II-like 1 ELISA Kit, ACVRL1 ELISA Kit, acvrl1 ELISA Kit, Acvrl1 ELISA Kit
基因ID
20568
UniProt
P97430
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