KIT Ligand ELISA 试剂盒
-
- 抗原 See all KIT Ligand (KITLG) ELISA试剂盒
- KIT Ligand (KITLG)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-2000 pg/mL
- 最低检测浓度
- 2 pg/mL
- 应用范围
- ELISA
- 原理
- Human SCF ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 2 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling KITLG ELISA Kit
- Top Product
- Discover our top product KITLG ELISA Kit
-
KIT Ligand (KITLG) ELISA Kit
KITLG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitSmall Sample KITLG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鸡 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitSmall Sample KITLG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 犬 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
KIT Ligand (KITLG) ELISA KitKITLG 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
KIT Ligand (KITLG) ELISA KitKITLG 适用: 小鼠 AA 26-190 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 - 5 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all KIT Ligand (KITLG) ELISA试剂盒
- KIT Ligand (KITLG)
- 别名
- SCF (KITLG 产品)
- 别名
- Xkl-1 ELISA Kit, Xsl ELISA Kit, Xsl-1 ELISA Kit, Xsl-2 ELISA Kit, steel ELISA Kit, KITLG ELISA Kit, SCF ELISA Kit, kitl ELISA Kit, kl-1 ELISA Kit, mgf ELISA Kit, scf ELISA Kit, FPH2 ELISA Kit, KL-1 ELISA Kit, Kitl ELISA Kit, MGF ELISA Kit, SF ELISA Kit, SHEP7 ELISA Kit, Clo ELISA Kit, Con ELISA Kit, Gb ELISA Kit, Kitlg ELISA Kit, Mgf ELISA Kit, SLF ELISA Kit, Sl ELISA Kit, blz ELISA Kit, contrasted ELISA Kit, 710-712 ELISA Kit, CSF ELISA Kit, KITL ELISA Kit, KIT ligand L homeolog ELISA Kit, KIT ligand ELISA Kit, kit ligand ELISA Kit, kitlg.L ELISA Kit, KITLG ELISA Kit, kitlg ELISA Kit, Kitl ELISA Kit, Kitlg ELISA Kit
- 背景
-
Gene Names: KITLG MGF SCF
Protein names: Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
- 基因ID
- 4254
- UniProt
- P21583
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
-
