IL1A ELISA 试剂盒
(8 references)Quick Overview for IL1A ELISA 试剂盒 (ABIN625017)
抗原
See all IL1A ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.5 pg/mL
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原理
- Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
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灵敏度
- 0.5 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 山羊 Colorimetric Sandwich ELISA 0.781 pg/mL - 50 pg/mL Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 大鼠 AA 115-270 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鸡 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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: "Topical Administration of Lactiplantibacillus plantarum (SkinDuoTM) Serum Improves Anti-Acne Properties." in: Microorganisms, Vol. 11, Issue 2, (2023) (PubMed).
: "Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6422-6, (2015) (PubMed).
: "Malignant small bowel obstruction: the last frontier for gastrointestinal stenting." in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1136-7, (2012) (PubMed).
: "Evaluating ESWL-induced renal injury based on urinary TNF-α, IL-1α, and IL-6 levels." in: Urological research, Vol. 40, Issue 5, pp. 569-73, (2012) (PubMed).
: "The protective effects of coumestrol against amyloid-β peptide- and lipopolysaccharide-induced toxicity on mice astrocytes." in: Neurological research, Vol. 33, Issue 6, pp. 663-72, (2012) (PubMed).
: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).
: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).
: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, (2008) (PubMed).
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: "Topical Administration of Lactiplantibacillus plantarum (SkinDuoTM) Serum Improves Anti-Acne Properties." in: Microorganisms, Vol. 11, Issue 2, (2023) (PubMed).
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL-1 alpha
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背景
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Gene Names: IL1A IL1F1
Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
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基因ID
- 3552
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UniProt
- P01583
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原 See all IL1A ELISA试剂盒
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