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IL1A ELISA 试剂盒

This 绵羊 IL1A ELISA Kit is a Colorimetric ELISA Kit designed to quantify 绵羊 IL1A.

产品编号 ABIN5690977

发货至: 中国

Quick Overview for IL1A ELISA 试剂盒 (ABIN5690977)

抗原

See all IL1A ELISA试剂盒

IL1A (Interleukin 1 alpha (IL1A))

适用

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绵羊

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Discover our top product IL1A ELISA Kit

原理

Ovine (sheep) IL-1 alpha/IL-1 F1 ELISA Kit.

Analytical Method

Quantitative

特异性

This ELISA antibody pair recognizes Ovine (sheep) IL-1 alpha.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 1 alpha (IL1A) ELISA Kit
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Interleukin 1 alpha (IL1A) ELISA Kit
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Interleukin 1 alpha (IL1A) ELISA Kit
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 大鼠 AA 115-270 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Serum

Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum

Interleukin 1 alpha (IL1A) ELISA Kit
High Sensitivity IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 小鸡 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: Hamster Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

4 °C

有效期

6 months
抗原 See all IL1A ELISA试剂盒

IL1A (Interleukin 1 alpha (IL1A))

别名

IL-1 alpha (IL-1 F1

基因ID

443404

UniProt

Q28579

途径

NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints

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