CYP27B1 ELISA 试剂盒
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- 抗原 See all CYP27B1 ELISA试剂盒
- CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0.29 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) ELISA Kit
CYP27B1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) ELISA KitCYP27B1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) ELISA KitCYP27B1 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) ELISA KitCYP27B1 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all CYP27B1 ELISA试剂盒
- CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
- 别名
- Cytochrome P450 27B1 (CYP27B1) (CYP27B1 产品)
- 别名
- cp2b ELISA Kit, cyp1 ELISA Kit, cyp1alpha ELISA Kit, cyp27b ELISA Kit, p450c1 ELISA Kit, pddr ELISA Kit, vdd1 ELISA Kit, vddr ELISA Kit, vddri ELISA Kit, vdr ELISA Kit, CP2B ELISA Kit, CYP1 ELISA Kit, CYP1alpha ELISA Kit, CYP27B ELISA Kit, P450c1 ELISA Kit, PDDR ELISA Kit, VDD1 ELISA Kit, VDDR ELISA Kit, VDDRI ELISA Kit, VDR ELISA Kit, Cyp40 ELISA Kit, Cp2b ELISA Kit, Cyp1 ELISA Kit, Cyp27b ELISA Kit, Pddr ELISA Kit, Vdd1 ELISA Kit, Vddr ELISA Kit, VddrI ELISA Kit, Vdr ELISA Kit, cytochrome P450 family 27 subfamily B member 1 ELISA Kit, cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily b, polypeptide 1 ELISA Kit, cyp27b1 ELISA Kit, CYP27B1 ELISA Kit, Cyp27b1 ELISA Kit
- 背景
- Alternative name: VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase
- 基因ID
- 1594
- UniProt
- O15528
- 途径
- Metabolism of Steroid Hormones and Vitamin D
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