CYP11A1 ELISA 试剂盒
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- 抗原 See all CYP11A1 ELISA试剂盒
- CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0133 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1) ELISA Kit
CYP11A1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1) ELISA KitCYP11A1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1) ELISA KitCYP11A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all CYP11A1 ELISA试剂盒
- CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
- 别名
- Cytochrome P450 11A1 (CYP11A1) (CYP11A1 产品)
- 别名
- CYP11A ELISA Kit, CYP11A1 ELISA Kit, CYPXIA1 ELISA Kit, P450SCC ELISA Kit, Cyp11a ELISA Kit, Cypxia1 ELISA Kit, D9Ertd411e ELISA Kit, P450scc ELISA Kit, Scc ELISA Kit, cscc ELISA Kit, P450(scc) ELISA Kit, SSC ELISA Kit, cytochrome P450 family 11 subfamily A member 1 ELISA Kit, cholesterol side-chain cleavage enzyme, mitochondrial-like ELISA Kit, cytochrome P450 cholesterol side-chain cleavage ELISA Kit, cytochrome P450, family 11, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 11, subfamily A, polypeptide 1 ELISA Kit, CYP11A1 ELISA Kit, LOC101827747 ELISA Kit, Cyp11a1 ELISA Kit
- 背景
- Alternative name: CYP11A, P450SCC, Cholesterol desmolase, Cholesterol Side Chain Cleavage Enzyme, Cytochrome P450 Family 11 Subfamily A Polypeptide 1
- 基因ID
- 1583
- UniProt
- P05108
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin
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