ATP6AP2 ELISA 试剂盒
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- 抗原 See all ATP6AP2 ELISA试剂盒
- ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0055 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all ATP6AP2 ELISA试剂盒
- ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))
- Abstract
- ATP6AP2 产品
- 别名
- APT6M8-9 ELISA Kit, ATP6IP2 ELISA Kit, ATP6M8-9 ELISA Kit, ELDF10 ELISA Kit, M8-9 ELISA Kit, MRXE ELISA Kit, MSTP009 ELISA Kit, XMRE ELISA Kit, (P)RR ELISA Kit, 5730403E06Rik ELISA Kit, Atp6ip2 ELISA Kit, tj6 ELISA Kit, wss ELISA Kit, arcl ELISA Kit, j6b7 ELISA Kit, stv1 ELISA Kit, tj6m ELISA Kit, tj6s ELISA Kit, vph1 ELISA Kit, atp6a2 ELISA Kit, atp6n1d ELISA Kit, ATP6AP2 ELISA Kit, DKFZp459G0918 ELISA Kit, atp6ap2 ELISA Kit, wu:fj36c03 ELISA Kit, wu:fq15e04 ELISA Kit, zgc:73194 ELISA Kit, ATPase H+ transporting accessory protein 2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 ELISA Kit, ATPase H+ transporting V0 subunit d2 ELISA Kit, ATPase H+ transporting V0 subunit a2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog ELISA Kit, ATP6AP2 ELISA Kit, Atp6ap2 ELISA Kit, ATP6V0D2 ELISA Kit, atp6v0a2 ELISA Kit, atp6ap2.S ELISA Kit, atp6ap2 ELISA Kit
- 背景
- Alternative name: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9
- 基因ID
- 70495
- UniProt
- Q9CYN9
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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