WNT10B CLIA Kit
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- 抗原 See all WNT10B CLIA Kits
- WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 312.5 pg/mL - 20000 pg/mL
- 最低检测浓度
- 312.5 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) and analogues was observed.
- 灵敏度
- 11.88 pg/mL
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Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit
WNT10B 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA KitWNT10B 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all WNT10B CLIA Kits
- WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))
- 别名
- Wingless Type MMTV Integration Site Family, Member 10B (WNT10B 产品)
- 别名
- WNT10B CLIA Kit, Xwnt-10b CLIA Kit, Xwnt10b CLIA Kit, wnt-12 CLIA Kit, Wnt12 CLIA Kit, SHFM6 CLIA Kit, WNT-12 CLIA Kit, Xwnt-10 CLIA Kit, wnt10 CLIA Kit, wnt10-A CLIA Kit, Zwnt[d] CLIA Kit, wnt10br CLIA Kit, wnt[d] CLIA Kit, Wnt family member 10B CLIA Kit, wingless-type MMTV integration site family, member 10B CLIA Kit, Wnt family member 10B S homeolog CLIA Kit, wingless-type MMTV integration site family, member 10b CLIA Kit, WNT10B CLIA Kit, wnt10b CLIA Kit, Wnt10b CLIA Kit, wnt10b.S CLIA Kit
- 背景
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Gene Name: Wingless Type MMTV Integration Site Family, Member 10B
Gene Aliases: WNT-12, SHFM6
- 基因ID
- 7480
- UniProt
- O00744
- 途径
- WNT signaling, Skeletal Muscle Fiber Development
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