p53 CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for p53 CLIA Kit (ABIN5659689)
抗原
See all p53 (TP53) CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78.12 pg/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Tumor Protein p53 (TP53). No significant cross-reactivity or interference between Tumor Protein p53 (TP53) and analogues was observed.
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灵敏度
- 9.5 pg/mL
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Tumor Protein P53 (TP53) ELISA Kit
TP53 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 acetylated Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 AA 1-393 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Lysate
Tumor Protein P53 (TP53) ELISA KitTP53 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 2 - 3 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Protein p53 (TP53). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Protein p53 (TP53). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Protein p53 (TP53) level in the sample or standard.,
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein p53 (TP53) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein p53 (TP53) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- Tumor Protein p53
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背景
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Gene Name: Tumor Protein p53
Gene Aliases: p53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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基因ID
- 7157
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UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原 See all p53 (TP53) CLIA Kits
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