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TNFSF13 ELISA 试剂盒

TNFSF13 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5659653
发货至: 中国
  • 抗原 See all TNFSF13 ELISA试剂盒
    TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    46.88 pg/mL - 3000 pg/mL
    最低检测浓度
    46.88 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13). No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) and analogues was observed.
    灵敏度
    16.53 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all TNFSF13 ELISA试剂盒
    TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
    别名
    Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13 产品)
    别名
    APRIL ELISA Kit, CD256 ELISA Kit, TALL-2 ELISA Kit, TALL2 ELISA Kit, TRDL-1 ELISA Kit, ZTNF2 ELISA Kit, 2310026N09Rik ELISA Kit, April ELISA Kit, Tall2 ELISA Kit, Trdl1 ELISA Kit, SLAN ELISA Kit, ANP32B ELISA Kit, april ELISA Kit, tnf13 ELISA Kit, TNLG7B ELISA Kit, TNF superfamily member 13 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13 ELISA Kit, acidic leucine-rich nuclear phosphoprotein 32 family member B ELISA Kit, TNFSF13 ELISA Kit, Tnfsf13 ELISA Kit, LOC494442 ELISA Kit, tnfsf13 ELISA Kit
    背景

    Gene Name: Tumor Necrosis Factor Ligand Superfamily, Member 13

    Gene Aliases: CD256, APRIL, TALL2, TRDL1, ZTNF2, A Proliferation Inducing Ligand, TNF And APOL Related Leukocyte Expressed Ligand 2, TNF-Related Death Ligand 1

    基因ID
    8741
    UniProt
    O75888
    途径
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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