TNFSF13 ELISA 试剂盒
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- 抗原 See all TNFSF13 ELISA试剂盒
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 抗原表位
- AA 105-250
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF13/APRIL
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
- Expression system for standard: NSO,A105-L250
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A105-L250 - Featured
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA Kit
TNFSF13 适用: 人 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 小鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 猴 AA 105-250 Colorimetric Sandwich ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) ELISA KitTNFSF13 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF13 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TNFSF13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFSF13 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 653, Standard deviation: 26.8, CV(%): 4.1
- Sample 2: n=16, Mean(pg/ml): 2945, Standard deviation: 156.1, CV(%): 5.3
- Sample 3: n=16, Mean(pg/ml): 5633, Standard deviation: 219.7, CV(%): 3.9,
- Sample 1: n=24, Mean(pg/ml): 864, Standard deviation: 42.4, CV(%): 4.9
- Sample 2: n=24, Mean(pg/ml): 3214, Standard deviation: 202.5, CV(%): 6.3
- Sample 3: n=24, Mean(pg/ml): 5366, Standard deviation: 268.3, CV(%): 5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "B-cell-activating factor, a proliferation inducing ligand and co-stimulatory molecules in the pathogenesis of immune thrombocytopenia in childhood." in: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, (2014) (PubMed).
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: "B-cell-activating factor, a proliferation inducing ligand and co-stimulatory molecules in the pathogenesis of immune thrombocytopenia in childhood." in: Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, (2014) (PubMed).
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- 抗原 See all TNFSF13 ELISA试剂盒
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 别名
- TNFSF13/APRIL (TNFSF13 产品)
- 别名
- APRIL ELISA Kit, CD256 ELISA Kit, TALL-2 ELISA Kit, TALL2 ELISA Kit, TRDL-1 ELISA Kit, ZTNF2 ELISA Kit, 2310026N09Rik ELISA Kit, April ELISA Kit, Tall2 ELISA Kit, Trdl1 ELISA Kit, SLAN ELISA Kit, ANP32B ELISA Kit, april ELISA Kit, tnf13 ELISA Kit, TNLG7B ELISA Kit, TNF superfamily member 13 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13 ELISA Kit, acidic leucine-rich nuclear phosphoprotein 32 family member B ELISA Kit, TNFSF13 ELISA Kit, Tnfsf13 ELISA Kit, LOC494442 ELISA Kit, tnfsf13 ELISA Kit
- 背景
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Background: Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation-inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.
Synonyms: Tumor necrosis factor (Ligand) superfamily member 13 transcript variant delta ,Tumor necrosis factor ligand superfamily member 13 ,TNFSF13 ,
Full Gene Name: tumor necrosis factor (ligand) superfamily, member 13
- 基因ID
- 8741
- UniProt
- Q2QBA2
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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