SERPINB6 ELISA 试剂盒
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- 抗原 See all SERPINB6 ELISA试剂盒
- SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Placental Thrombin Inhibitor (PTI). No significant cross-reactivity or interference between Placental Thrombin Inhibitor (PTI) and analogues was observed.
- 灵敏度
- 0.059 ng/mL
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serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6) ELISA Kit
SERPINB6 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placental Thrombin Inhibitor (PTI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placental Thrombin Inhibitor (PTI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placental Thrombin Inhibitor (PTI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placental Thrombin Inhibitor (PTI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placental Thrombin Inhibitor (PTI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SERPINB6 ELISA试剂盒
- SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
- 别名
- Placental Thrombin Inhibitor (SERPINB6 产品)
- 别名
- Serpinb6a ELISA Kit, cap ELISA Kit, dfnb91 ELISA Kit, mstp057 ELISA Kit, pi6 ELISA Kit, pti ELISA Kit, serpinb8 ELISA Kit, spi3 ELISA Kit, 4930482L21Rik ELISA Kit, D330015H01Rik ELISA Kit, PI-6 ELISA Kit, Serpinb6 ELISA Kit, Spi3 ELISA Kit, ovalbumin ELISA Kit, CAP ELISA Kit, DFNB91 ELISA Kit, MSTP057 ELISA Kit, PI6 ELISA Kit, PTI ELISA Kit, SPI3 ELISA Kit, serpin family B member 6A ELISA Kit, serpin family B member 6 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6a ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 6 ELISA Kit, Serpinb6a ELISA Kit, serpinb6 ELISA Kit, SERPINB6 ELISA Kit
- 背景
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Gene Name: Placental Thrombin Inhibitor
Gene Aliases: SERPINB6, CAP, PI6, SPI3, Serpin Peptidase Inhibitor Clade B Member 6, Cytoplasmic antiproteinase, Peptidase inhibitor 6
- 基因ID
- 5269
- UniProt
- P35237
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