SERPINB6 ELISA 试剂盒
SERPINB6
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
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- 抗原 See all SERPINB6 ELISA试剂盒
- SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Serpin B6 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6) ELISA Kit
SERPINB6 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all SERPINB6 ELISA试剂盒
- SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))
- 别名
- Serpin B6 (SERPINB6 产品)
- 别名
- Serpinb6a ELISA Kit, cap ELISA Kit, dfnb91 ELISA Kit, mstp057 ELISA Kit, pi6 ELISA Kit, pti ELISA Kit, serpinb8 ELISA Kit, spi3 ELISA Kit, 4930482L21Rik ELISA Kit, D330015H01Rik ELISA Kit, PI-6 ELISA Kit, Serpinb6 ELISA Kit, Spi3 ELISA Kit, ovalbumin ELISA Kit, CAP ELISA Kit, DFNB91 ELISA Kit, MSTP057 ELISA Kit, PI6 ELISA Kit, PTI ELISA Kit, SPI3 ELISA Kit, serpin family B member 6A ELISA Kit, serpin family B member 6 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6a ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 6 ELISA Kit, Serpinb6a ELISA Kit, serpinb6 ELISA Kit, SERPINB6 ELISA Kit
- 基因ID
- 5269
- UniProt
- P35237
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