C1S CLIA Kit
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- 抗原 See all C1S CLIA Kits
- C1S (Complement Component 1, S Subcomponent (C1S))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 468.75 pg/mL - 30000 pg/mL
- 最低检测浓度
- 468.75 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Complement Component 1, S Subcomponent (C1s). No significant cross-reactivity or interference between Complement Component 1, S Subcomponent (C1s) and analogues was observed.
- 灵敏度
- 18.22 pg/mL
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Complement Component 1, S Subcomponent (C1S) ELISA Kit
C1S 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, S Subcomponent (C1s). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 1, S Subcomponent (C1s). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 1, S Subcomponent (C1s) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all C1S CLIA Kits
- C1S (Complement Component 1, S Subcomponent (C1S))
- 别名
- Complement Component 1, S Subcomponent (C1S 产品)
- 别名
- AA959438 CLIA Kit, AI255193 CLIA Kit, AI327365 CLIA Kit, C1sa CLIA Kit, r-gsp CLIA Kit, C1S CLIA Kit, complement C1s CLIA Kit, complement component 1, s subcomponent 1 CLIA Kit, complement component 1, s subcomponent CLIA Kit, complement component 1, s subcomponent L homeolog CLIA Kit, complement C1s subcomponent CLIA Kit, C1S CLIA Kit, C1s1 CLIA Kit, C1s CLIA Kit, c1s CLIA Kit, c1s.L CLIA Kit, LOC100558615 CLIA Kit
- 背景
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Gene Name: Complement Component 1, S Subcomponent
Gene Aliases: C1 esterase
- 基因ID
- 716
- UniProt
- P09871
- 途径
- Complement System
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