Retinoblastoma 1 ELISA 试剂盒
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- 抗原 See all Retinoblastoma 1 (RB1) ELISA试剂盒
- Retinoblastoma 1 (RB1)
- 抗原表位
- pThr826, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-RB (Thr826) and Total RB ELISA Kit. This assay semi-quantitatively measures RB phosphorylated at Threonine-826 as well as total RB in cell lysate samples.
- 样品类型
- Cell Culture Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human RB phosphorylated at site Threonine-826 as well as total RB.
- 产品特性
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- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Retinoblastoma 1 (RB1) ELISA Kit
RB1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Retinoblastoma 1 (RB1) ELISA KitRB1 适用: 人 pThr826 Colorimetric Sandwich ELISA Cell Culture Lysate
Retinoblastoma 1 (RB1) ELISA KitRB1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Retinoblastoma 1 (RB1) ELISA试剂盒
- Retinoblastoma 1 (RB1)
- 别名
- RB (RB1 产品)
- 别名
- OSRC ELISA Kit, RB ELISA Kit, p105-Rb ELISA Kit, pRb ELISA Kit, pp110 ELISA Kit, Rb ELISA Kit, Rb-1 ELISA Kit, P105-RB ELISA Kit, PP110 ELISA Kit, RB11 ELISA Kit, zgc:154147 ELISA Kit, RB transcriptional corepressor 1 ELISA Kit, RB transcriptional corepressor like 1 ELISA Kit, retinoblastoma 1 ELISA Kit, RB1 ELISA Kit, RBL1 ELISA Kit, Rb1 ELISA Kit, rb1 ELISA Kit
- 基因ID
- 5925
- UniProt
- P06400
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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