HMGB1 ELISA 试剂盒
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- 抗原 See all HMGB1 ELISA试剂盒
- HMGB1 (High Mobility Group Box 1 (HMGB1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 原理
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 18.75 pg/mL
- 组件
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling HMGB1 ELISA Kit
- Top Product
- Discover our top product HMGB1 ELISA Kit
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- 样本量
- 100 µL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- 试剂准备
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL working solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
- 储存方法
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma." in: Biomaterials, Vol. 181, pp. 35-52, (2018) (PubMed).
: "Harnessing the cross-talk between tumor cells and tumor-associated macrophages with a nano-drug for modulation of glioblastoma immune microenvironment." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 268, pp. 128-146, (2018) (PubMed).
: "Profiling of Canonical and Non-Traditional Cytokine Levels in Interferon-β-Treated Relapsing-Remitting-Multiple Sclerosis Patients." in: Frontiers in immunology, Vol. 9, pp. 1240, (2018) (PubMed).
: "Young alcohol binge drinkers have elevated blood endotoxin, peripheral inflammation and low cortisol levels: neuropsychological correlations in women." in: Addiction biology, Vol. 23, Issue 5, pp. 1130-1144, (2018) (PubMed).
: "Short-Term Postoperative Cognitive Dysfunction and Inflammatory Response in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy: A Pilot Study." in: Mediators of inflammation, Vol. 2017, pp. 3605350, (2018) (PubMed).
: "Relationships of BRAF mutation and HMGB1 to papillary thyroid carcinoma." in: Biochemical and biophysical research communications, Vol. 486, Issue 4, pp. 898-903, (2017) (PubMed).
: "Blood purification treatment initiated at the time of sepsis diagnosis effectively attenuates serum HMGB1 upregulation and improves patient prognosis." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3029-3035, (2017) (PubMed).
: "HMGB1 Enhances Drug Resistance and Promotes In Vivo Tumor Growth of Lung Cancer Cells." in: DNA and cell biology, Vol. 35, Issue 10, pp. 622-627, (2016) (PubMed).
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Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma." in: Biomaterials, Vol. 181, pp. 35-52, (2018) (PubMed).
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- 抗原 See all HMGB1 ELISA试剂盒
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 别名
- HMGB-1(High mobility group protein B1) (HMGB1 产品)
- 别名
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- 途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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