IFI16
(Interferon, gamma-Inducible Protein 16 (IFI16))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
The IFI16 (Gamma-interferon-inducible protein 16) ELISA Kit (Human) is a quantitative sandwich assay for the detection of IFI16 in human serum, plasma, tissue homogenates and other biological fluids.
样品类型
Plasma, Serum, Tissue Homogenate
Analytical Method
Quantitative
灵敏度
> 0.094 ng/mL
产品特性
IFI16 (Gamma-interferon-inducible protein 16) ELISA Kit (Human)
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFI16 . Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to IFI16. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFI16, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IFI16 in the sample is then determined by comparing the O.D. of the sample to the standard curve.