The Hmgb1 (High mobility group protein B1) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids.
样品类型
Plasma, Serum, Tissue Homogenate
Analytical Method
Quantitative
灵敏度
9.375 pg/mL
产品特性
Hmgb1 (High mobility group protein B1) ELISA Kit (Mouse)
This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to Hmgb1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hmgb1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hmgb1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.