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HMGB1 ELISA 试剂盒

HMGB1 适用: 小鼠 Colorimetric Sandwich ELISA Plasma, Serum, Tissue Homogenate
产品编号 ABIN5067936
发货至: 中国
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    HMGB1 (High Mobility Group Box 1 (HMGB1))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    The Hmgb1 (High mobility group protein B1) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids.
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    9.375 pg/mL
    产品特性
    Hmgb1 (High mobility group protein B1) ELISA Kit (Mouse)
    组件
    • *356299A: Microtiter Strips, 1x96 wells (8x12 wells).
    • *356299B: Standard, 2x1 vial
    • 356299C: Sample/Standard Dilution Buffer, 1x20ml
    • 356299D: Antibody (Biotin) (Concentrated), 1x120ul
    • 356299E: Antibody Dilution Buffer, 1x10ml
    • 356299F: Streptavidin (HRP) (SABC), 1x120ul
    • 356299G: SABC Dilution Buffer, 1x10ml
    • 356299H: TMB Substrate, 1x10ml
    • 356299J: Stop Solution, 1x10ml
    • 356299K: Wash Buffer, 25X, 1x30ml
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  • 板类型
    Pre-coated
    实验流程
    Principle:
    • This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to Hmgb1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hmgb1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hmgb1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
    实验精密度
    Precision:
    • Intra-assay CV: <8%
    • Inter-assay CV: <10%
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    4°C/-20°C
  • 抗原 See all HMGB1 ELISA试剂盒
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    别名
    Hmgb1 (High mobility group protein B1) (HMGB1 产品)
    别名
    HMG1 ELISA Kit, HMG3 ELISA Kit, SBP-1 ELISA Kit, DEF ELISA Kit, HMG-1 ELISA Kit, Hmg1 ELISA Kit, amphoterin ELISA Kit, p30 ELISA Kit, hmgb1 ELISA Kit, ik:tdsubc_1a5 ELISA Kit, wu:fb23c02 ELISA Kit, xx:tdsubc_1a5 ELISA Kit, zgc:56110 ELISA Kit, zgc:77104 ELISA Kit, hmg-1 ELISA Kit, hmg3 ELISA Kit, sbp-1 ELISA Kit, hmg1 ELISA Kit, HMGB1 ELISA Kit, Ac2-008 ELISA Kit, high mobility group box 1 ELISA Kit, high-mobility group box 1 ELISA Kit, high mobility group box 1a ELISA Kit, high mobility group box 1 L homeolog ELISA Kit, high mobility group protein B1 ELISA Kit, HMGB1 ELISA Kit, Hmgb1 ELISA Kit, hmgb1 ELISA Kit, hmgb1a ELISA Kit, hmgb1.L ELISA Kit, LOC100359149 ELISA Kit
    途径
    p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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