C4A ELISA 试剂盒
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- 抗原 See all C4A (C4a) ELISA试剂盒
- C4A (C4a) (Complement Fragment C4a (C4a))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- The Complement C4-A (C4A) ELISA Kit (Human) is a quantitative sandwich assay for the detection of C4A in human serum, plasma, tissue homogenates and other biological fluids.
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- > 0.938 ng/mL
- 产品特性
- Complement C4-A (C4A) ELISA Kit (Human)
- 组件
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- *354394A: Microtiter Strips, 8x12 wells.
- *354394B: Standard, 2 vials
- 354394C: Sample/Standard Dilution Buffer, 1x20ml
- 354394D: Antibody-Biotin (Concentrated), 1x120ul
- 354394E: Antibody Dilution Buffer, 1x10ml
- 354394F: Streptavidin-HRP Conjugate (SABC), 1x120ul
- 354394G: SABC Dilution Buffer, 1x10ml
- 354394H: TMB Substrate, 1x10ml
- 354394J: Stop Solution, 1x10ml
- 354394K: Wash Buffer (25X), 1x30ml
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Complement Fragment C4a (C4a) ELISA Kit
C4a 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment C4a (C4a) ELISA KitC4a 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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- 板类型
- Pre-coated
- 实验流程
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Principle:
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4A. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to C4A. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4A, biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4A in the sample is then determined by comparing the O.D. of the sample to the standard curve.
- 实验精密度
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C/-20°C
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- 抗原 See all C4A (C4a) ELISA试剂盒
- C4A (C4a) (Complement Fragment C4a (C4a))
- 别名
- Complement C4-A (C4A) (C4a 产品)
- 别名
- C4 ELISA Kit, C4A2 ELISA Kit, C4A3 ELISA Kit, C4A4 ELISA Kit, C4A6 ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CO4 ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, Slp ELISA Kit, C4-1 ELISA Kit, C4b ELISA Kit, complement C4A (Rodgers blood group) ELISA Kit, complement component 4A (Rodgers blood group) ELISA Kit, C4A ELISA Kit, C4a ELISA Kit
- 途径
- Complement System
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