IL1A ELISA 试剂盒
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Quick Overview for IL1A ELISA 试剂盒 (ABIN4986939)
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See all IL1A ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.02 ng/mL
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant Rat IL-1α Ligand
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灵敏度
- 7 pg/mL
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试剂未包括
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- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
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Interleukin 1 alpha (IL1A) ELISA Kit
IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 山羊 Colorimetric Sandwich ELISA 0.781 pg/mL - 50 pg/mL Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 犬 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 大鼠 AA 115-270 Colorimetric Sandwich ELISA 4.69-300 pg/mL Cell Culture Supernatant, Serum
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 人 Colorimetric Sandwich ELISA 3.906 pg/mL - 250 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 1 alpha (IL1A) ELISA KitHigh Sensitivity IL1A 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1 alpha (IL1A) ELISA KitIL1A 适用: 小鸡 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Detection Wavelength: 450 nm
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样本量
- 20 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL1A (Interleukin 1 alpha (IL1A))
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别名
- IL-1alpha
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背景
- Interleukin-1α (IL-1α, also known as IL-1F1) and IL-1β (IL-1F2) are pleiotropic cytokines that belong to the IL-1 gene family. IL-1α and IL-1β bind to the same cell surface receptors and share biological functions. The two proteins have approximately 23 % amino acid (aa) sequence homology and both are synthesized as 31 kDa precursors that lack hydrophobic signal peptide sequences. Current evidence suggests that IL-1 proteins may be secreted via non-classical pathways (1-4).Rat IL-1α cDNA encodes a 270 aa residue pro-IL-1α precursor (5). The 114 aa pro-region contains a nuclear localization sequence, a lysine-based myristoylation site, one phosphorylation site, and one potential N-linked glycosylation site (5-8). The 156 aa mature region contains no cysteines and one potential N-linked glycosylation site (5). Pro-IL-1α is primarily localized to the cytosol after synthesis. It is known to translocate to the nucleus after cell activation and initiate gene transcription (9, 10). Some IL-1α is released extracellularly and exists as either a membrane-bound form or as a circulating 17 kDa molecule. When membranebound, membrane association is mediated either via a poorly-understood cell surface lectin interaction with the glycosylated IL-1α pro-form, or by interaction of myristoylated pro-IL-1α with plasma membrane phospho-lipids (8, 11-13). When released as a 17 kDa soluble form, calpain initiates cleavage of the pro-IL-1α precursor (14). Unlike pro-IL-1β, which is biologically inactive, both pro-IL-1α and mature IL-1α have been shown to be biologically active (15, 16). Within the mature protein, rat IL-1α shares 79 %, 60 %, 59 %, 58 %, 57 %, and 56 % aa sequence identity with mouse, rabbit, human, bovine, canine and feline proteins, respectively (17-21). Mammalian cells known to express IL-1α include brown adipocytes (22), keratinocytes (23), monocytes (24), macrophages (25), endothelial and smooth muscle cells (26), mast cells (27), Schwann cells (28), as well as osteoblasts and osteoclasts (29).Three type I transmembrane immunoglobulin superfamily proteins, IL-1 receptor type I (IL-1 R1), IL-1 receptor type II (IL-1 R2), and IL-1 receptor accessory protein (IL-1 RAcP, IL-1 R3)are involved in the formation of high affinity cell surface IL-1 receptor complexes. Both IL-1 R1 and IL-1 R2 can bind directly to IL-1α. IL-1 RAcP does not bind IL-1α directly, but interacts with IL-1 R1 in the presence of IL-1 to form the high-affinity receptor complex which is required for intracellular signal transduction. IL-1 RAcP also interacts with IL-1 R2 to form a non-functional high-affinity receptor complex that does not transduce IL-1 signals. Therefore, IL-1 R2 functions as a decoy receptor that attenuates IL-1α functions (16, 30, 31).IL-1α possesses a wide variety of biological activities and plays a central role in mediating immune and inflammatory responses. Normal production of IL-1α is critical for hematopoiesis, angiogenesis, osteoclast differentiation, and initiation of normal host responses to injury and infection(15,32-34). Inappropriate production of IL-1α has been implicated in the production of a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, insulin-dependent diabetes mellitus, and atherosclerosis (1, 13, 15).
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途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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