STAT1 ELISA 试剂盒
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- 抗原 See all STAT1 ELISA试剂盒
- STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Human Phosphotyrosine STAT1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine STAT1 in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-STAT1.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA Kit
STAT1 适用: 人, 小鼠, 大鼠 pTyr701 Colorimetric Cell ELISA Cell Culture Cells
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人, 小鼠 pSer727 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人, 小鼠 pTyr701 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人, 小鼠 pSer727 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) ELISA KitSTAT1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all STAT1 ELISA试剂盒
- STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
- 别名
- STAT 1 (STAT1 产品)
- 别名
- CANDF7 ELISA Kit, ISGF-3 ELISA Kit, STAT91 ELISA Kit, 2010005J02Rik ELISA Kit, AA408197 ELISA Kit, XStat1 ELISA Kit, STAT4 ELISA Kit, DD6G4-4 ELISA Kit, STAT ELISA Kit, STAT1 ELISA Kit, dZ182H3.3 ELISA Kit, si:by134g18.2 ELISA Kit, si:by51f19.1 ELISA Kit, si:dz182h3.3 ELISA Kit, si:dz199m19.1 ELISA Kit, si:dz73o4.2 ELISA Kit, stat1 ELISA Kit, signal transducer and activator of transcription 1 ELISA Kit, signal transducer and activator of transcription 1 L homeolog ELISA Kit, signal transducer and activator of transcription 1, 91kDa ELISA Kit, signal transducer and activator of transcription 4 ELISA Kit, signal transducer and activator of transcription 1a ELISA Kit, STAT1 ELISA Kit, Stat1 ELISA Kit, stat1.L ELISA Kit, stat4 ELISA Kit, stat1a ELISA Kit
- 基因ID
- 6772
- UniProt
- P42224
- 途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
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