Selectin E/CD62e ELISA 试剂盒
-
- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Mouse E-Selectin SpeedELISA Kit.
- 品牌
- SpeedELISA
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 产品特性
-
- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling SELE ELISA Kit
- Top Product
- Discover our top product SELE ELISA Kit
-
Selectin E (SELE) ELISA Kit
SELE 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Selectin E (SELE) ELISA KitSELE 适用: 犬 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 AA 22-556 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Selectin E (SELE) ELISA KitSELE 适用: 小鼠 Soluble Colorimetric 78-5000 pg/mL Plasma, Serum, Tissue Homogenate
Selectin E (SELE) ELISA KitSELE 适用: 人 Soluble Colorimetric Sandwich ELISA 93.50-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 样本量
- 100 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
-
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - 有效期
- 6 months
-
- 抗原 See all Selectin E/CD62e (SELE) ELISA试剂盒
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- 别名
- E-Selectin (SELE 产品)
- 别名
- SELE ELISA Kit, CD62E ELISA Kit, ELAM ELISA Kit, ELAM1 ELISA Kit, ESEL ELISA Kit, LECAM2 ELISA Kit, E-selectin ELISA Kit, Elam ELISA Kit, selectin E ELISA Kit, selectin, endothelial cell ELISA Kit, SELE ELISA Kit, Sele ELISA Kit
- 基因ID
- 20339
- UniProt
- Q00690
- 途径
- Thromboxane A2 Receptor Signaling
-
