TRKA ELISA 试剂盒
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- 抗原 See all TRKA (NTRK1) ELISA试剂盒
- TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-500 ng/mL
- 最低检测浓度
- 2 ng/mL
- 应用范围
- ELISA
- 原理
- Human TrkA ELISA Kit for serum, plasma, and cell culture supernatant samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human TrkA. Other species not determined.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling NTRK1 ELISA Kit
- Top Product
- Discover our top product NTRK1 ELISA Kit
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Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kit
NTRK1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA KitNTRK1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA KitNTRK1 适用: 大鼠 AA 33-418 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA KitNTRK1 适用: 人 AA 33-407 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all TRKA (NTRK1) ELISA试剂盒
- TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
- 别名
- TrkA (NTRK1 产品)
- 别名
- MTC ELISA Kit, TRK ELISA Kit, TRK1 ELISA Kit, TRKA ELISA Kit, Trk-A ELISA Kit, p140-TrkA ELISA Kit, CDHF12 ELISA Kit, CDHR16 ELISA Kit, HSCR1 ELISA Kit, MEN2A ELISA Kit, MEN2B ELISA Kit, MTC1 ELISA Kit, PTC ELISA Kit, RET-ELE1 ELISA Kit, RET51 ELISA Kit, Trk ELISA Kit, C80751 ELISA Kit, Tkr ELISA Kit, TrkA ELISA Kit, trk ELISA Kit, trka ELISA Kit, CTRKA ELISA Kit, trkA ELISA Kit, neurotrophic receptor tyrosine kinase 1 ELISA Kit, ret proto-oncogene ELISA Kit, neurotrophic tyrosine kinase, receptor, type 1 ELISA Kit, NTRK1 ELISA Kit, RET ELISA Kit, Ntrk1 ELISA Kit, ntrk1 ELISA Kit
- 基因ID
- 4914
- UniProt
- P04629
- 途径
- RTK signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process
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